蓝莓视频

Imperial researchers demonstrated听蝉耻颈迟补产颈濒颈迟测听补苍诲听robustness听of听specialised听spectroscopy for听molecular听analysis听of human听samples in a multi-laboratory trial.听

The Imperial College Phenome Centre (IPC) – the core facility of the 蓝莓视频 Imperial BRC Molecular Phenomics Theme – led a multi-centre laboratory study to assess the precision and accuracy of measuring lipoprotein concentrations by NMR spectroscopy in human samples.

Lipoproteins are classified based on their density and size, into high-, low- and very low-density lipoproteins, and chylomicrons, which are composed of dietary fat triglycerides. Circulating lipoproteins听in blood, more commonly known as 鈥榗holesterol levels鈥�, are听routinely measured in clinic and are used as an indicator of cardiovascular and other disorders. However standard examination has limitations with respect to turnaround time and the depth of information it provides.

Dr Beatriz Jim茅nez, NMR Manager at the IPC and lead author of the study, explained further: 鈥淣MR provides a more detailed picture than the routine laboratory analysis. It is also quick and relatively cheap. In a routine clinical lab only 3 parameters are measured. Alternatively,听a听specialised听lab can provide a similar听in-depth听analysis to the one obtained by NMR, however it takes almost 1 week per sample and is quite laborious. NMR allows the measurement of 112 relevant lipoprotein subfractions, or听鈥�cholesterol levels鈥�,听with only a 4-minute experiment.鈥�

Scientists听from five laboratories in three institutions听analysed听98听blood serum and plasma听samples听using 11 NMR spectrometers听following听the same protocols, while operators were not exchanged between听labs, even within same institutions.听Study results,听published in ,听demonstrated听exceptional听reproducibility of lipoprotein听quantification听measured by NMR, compliant听with NCEP听requirements听(National Cholesterol Education Program) for the measurement of lipids in certified clinical laboratories. In addition to lipoprotein听amounts, quantification of 26 metabolites,听including glucose, lactate, several amino听acids and creatinine,听was听also possible by using the same 4–minute NMR measurement,听with negligible analytical听variation. However, the authors do caution that lower molecular weight metabolites would need to be considered on individual basis.听

As with most medical tests, inter-institutional reproducibility is crucial to avoid operator bias and to ensure wider NHS implementation.听This work provides important evidence for reliable and reproducible use of听NMR spectroscopy in clinic, which can provide informative in-depth biochemical information听to aid clinical decision-making at patient level in most disorders where lipid metabolism is deregulated.

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Molecular听Phenomics听is an听蓝莓视频 Imperial BRC Cross-cutting Theme and forms part of ITMAT.听听